RESUMO: Este estudo objetivou realizar uma análise bibliométrica e revisão de literatura acerca da produção de filmes à base de gelatina para aplicação como embalagens alimentícias, abordando os principais avanços e limitações. A busca de artigos foi realizada na base de dados da Scopus e os dados bibliométricos obtidos pela ferramenta Bibliometrix (RStudio software). Verificou-se uma grande variedade de compostos que podem ser incorporados nos filmes de gelatina, a fim de superar suas limitações relacionadas à alta solubilidade e baixas propriedades mecânicas, bem como para obtenção de funções ativas ou inteligentes. Dentre os compostos mais reportados, têm-se: óleos essenciais, pigmentos extraídos de vegetais e outros agentes antimicrobianos. Os alimentos mais reportados como matriz de aplicação foram: carnes (peixe, frango e camarão), leite, queijo e frutas, minimamente processadas. Mesmo com tendências promissoras, o maior desafio no âmbito das aplicações reais em larga escala é a obtenção de biopolímeros facilmente degradáveis, com estabilidade estrutural e funcional similar aos polímeros sintéticos. Dessa forma, o maior enfoque dessa temática em pesquisas poderá favorecer avanços significativos para o uso dessas embalagens, impactando positivamente diversos dos Objetivos para o Desenvolvimento Sustentável, preconizados pela Organização das Nações Unidas.

ABSTRACT: This research carried out a bibliometric analysis and literature review on the production of gelatin-based films for application as food packaging, addressing the main advances and limitations. The search for articles was performed in the Scopus database, and bibliometric data were obtained using the Bibliometrix tool (RStudio software). It was observed that a wide variety of compounds can be incorporated into gelatin films to overcome the limitations related to their high solubility and low mechanical properties, as well as to obtain active or smart functions. Among the most reported compounds were essential oils, pigments extracted from vegetables, and other antimicrobial agents. The most reported foods as an application matrix were meat (fish, chicken, and shrimp), milk, cheese, and minimally processed fruits. Even with promising trends, the biggest challenge for large-scale applications is to obtain easily degradable biopolymers with structural and functional stability similar to synthetic polymers. Thus, a greater focus on this theme in research may favor significant advances in the use of these packages and positively impact several of the Sustainable Development Goals, as recommended by the United Nations.

ABSTRACT Tuber floridanum is the first truffle species reported in Brazil and little is known about its nutritional properties and ecology. Therefore, this study aimed to (1) determine T. floridanum aroma and nutritional properties and (2) analyze cultivation and fruiting conditions, including potential crop pests and market value. Fruiting bodies of T. floridanum showed a slightly ellipsoid shape and smooth to shallow groovy surface. The mass of ascocarps ranged from 0.13 g to 15.95 g, and the truffle was composed of 71 % carbohydrates, 19 % protein, 5 % ash, and 5 % of fat. Octan-3-one, 1-methoxy-3-methyl-butane, and 1-methoxy-2-methyl-butane determined T. floridanum characteristic aroma. Soil properties were also specific for this truffle, differing from optimal soils where commercial truffles are produced. In addition, the occurrence of earwigs (Anisolabididae, Anisolabidinae) feeding on truffles (26 % of all ascocarps) in pecan orchards may reduce truffle quality and cause financial losses. T. floridanum aroma has a unique composition, completely different from any commercial and non-commercial truffle species analyzed so far. Soil conditions in pecan orchards were also specific for truffle fruiting. Further studies should elucidate other ecological conditions for the co-production of T. floridanum with pecan .

ABSTRACT Greek yogurt (GY) has gained popularity in recent years for its marked texture, taste, and nutritional characteristics compared to traditional yogurt (TY). The objective of this work was to analyze the physicochemical, sensory, and lipid profile of GY and TY with blueberry flavor, both manufactured by a local industry in the state of Rio Grande do Sul, Brazil. Protein and lipid content, as well as humidity, ash, and fatty acid profile were quantified and a sensory evaluation was completed using the affective method. The physicochemical results showed 1.5% and 2.3% more proteins and lipids, respectively, for GY compared to TY. The humidity in TY was 10% lower than in GY. Eighteen types of polyunsaturated, saturated, monounsaturated fatty acids were identified, with a high proportion of C14, C16, and C18. Sensory analysis showed a preference for GY over TY (64% versus 36%, p<0.05). However, body, appearance, and texture attributes did not present better acceptance scores for GY (p>0.05). Both the protein and lipid content, associated with creaminess, likely influence better acceptance of GY.

RESUMEN El yogur griego (YG) ha ganado popularidad durante los últimos años por su marcada textura, sabor y características nutricionales en comparación con el yogur tradicional (YT). El objetivo de este trabajo fue analizar el perfil fisicoquímico, sensorial y lipídico de YG y YT con sabor a arándano, ambos fabricados por una industria ubicada en el estado de Rio Grande do Sul, Brasil. Fueron cuantificados el contenido de proteínas, lípidos, humedad y cenizas, así como también el perfil de ácidos grasos y la evaluación sensorial por método afectivo. Los resultados fisicoquímicos mostraron que YG contiene 1,5% y 2,3% más de proteínas y lípidos, respectivamente, en comparación con YT (p<0,05). La humedad en YT fue 1,1 veces menor que en YG. Fueron identificados 18 tipos de ácidos grasos poliinsaturados, saturados, monoinsaturados, con mayor proporción de C14, C16 y C18. El análisis sensorial mostró una preferencia por YG de 64% sobre YT 36% (p<0,05). Sin embargo, no hubo diferencia significativa (p>0,05) en relación a la aceptación de los atributos color, olor, sabor y acidez. Los atributos cuerpo, apariencia y textura presentaron mejores scores de aceptación para el YG. Tanto el contenido de proteínas y lípidos, asociados a la cremosidad, probablemente hayan influenciado una mejor aceptación del YG.

Resumo O consumo e, consequentemente, a produção de manteiga têm aumentado consideravelmente nos últimos anos. Para conhecer as manteigas comercializadas no Brasil, o estudo teve como objetivo analisar manteigas de origem brasileira, francesa, italiana e argentina. As amostras foram analisadas quanto ao teor de gordura, à umidade, ao extrato seco desengordurado e à acidez total, para comparação com a legislação brasileira. Também foram determinados os teores de cloretos, proteínas, cinzas, extrato seco total, atividade de água, cor e perfil lipídico. Em todas as análises, houve diferença significativa entre as amostras. Algumas amostras apresentaram desacordo com os requisitos de qualidade recomendados pela legislação brasileira para comercialização. Dez amostras não apresentaram o teor mínimo de gordura exigido, variando de 68,53% a 77,31% nas manteigas com sal e de 71,64% a 81,72% nas manteigas sem sal. Oito amostras estavam acima do permitido para umidade, variando de 17,05% a 20,28%, e todas estavam de acordo em relação à acidez. Os ácidos graxos predominantes em todas as amostras foram ácido mirístico (C14:0), ácido palmítico (C16:0), ácido esteárico (C18:0) e ácido oleico (C18:1n9). A influência regional, a raça do animal, o manejo dos animais e a época do ano em que o leite foi obtido para a produção das manteigas são considerados os principais motivos das diferenças físico-químicas encontradas entre as amostras.

Abstract The consumption and consequent production of butter has increased considerably in recent years. In order to know the butters sold in Brazil, the study aimed to analyze butters of Brazilian, French, Italian and Argentine origin. The samples were analyzed for fat content, moisture, Defatted Dry Extract (DDE), and total acidity for comparison with Brazilian legislation. The levels of chlorides, protein, ash, total dry extract, water activity, color and determination of the lipid profile were also determined. In all analyses, there was a statistically significant difference between the samples. Some samples showed disagreement with the quality requirements recommended in Brazilian legislation for commercialization. Indeed, 10 samples did not present the minimum fat content required, varying from 68.53% to 77.31% in butters with salt and from 71.64% to 81.72% in those without salt. Eight samples presented humidity levels above the legal recommendations, varying from 17.05% to 20.28%. All products were in agreement with acidity levels. The predominant fatty acids in all samples were myristic acid (C14: 0), palmitic acid (C16: 0), stearic acid (C18: 0) and oleic acid (C18: 1n9). The regional influence, breed and handling of the animals and season of the year in which the milk was obtained for butter production, were suggested to be the main reason for the physical-chemical difference found between the samples.

Resumo Estima-se que o número global de pessoas afetadas pela deficiência de micronutrientes, também conhecida mundialmente como fome oculta, seja superior a dois bilhões, sendo assim considerada um problema de saúde pública. O objetivo deste estudo foi avaliar cultivares biofortificadas de mandioca quanto a composição físico-química e teor de carotenoides totais, além de verificar sua aceitação sensorial por escolares. O estudo foi realizado em Santa Maria-RS, Brasil, com quatro cultivares de mandioca, sendo duas variedades biofortificadas com polpa amarela (BRS 399 e BRS 396) e duas variedades não biofortificadas: uma com polpa amarela (“Gema de ovo”) e outra com polpa branca (“Vassourinha”). Após o cozimento das raízes, a composição nutricional (umidade, proteína, lipídio, cinzas e amido total), o teor de carotenoides totais e a aceitação sensorial das raízes pelas crianças, nos municípios sul-rio-grandenses de em Júlio de Castilhos e Dilermando de Aguiar, foram determinados. Os teores de umidade, proteína, lipídios, cinzas e amido apresentaram variações significativas entre as cultivares analisadas. Os maiores níveis de carotenoides totais foram encontrados nas cultivares biofortificadas (12,85 µg g-1). Nas cultivares não biofortificadas, “Gema de ovo” e “Vassourinha”, os níveis foram de 12,01 µg g-1 e 3,30 µg g-1, respectivamente. A aceitação sensorial foi de 78,7%, demonstrando potencial para inserção de raízes biofortificadas na alimentação escolar.

Abstract It is estimated that the global number of people affected by micronutrient deficiency, known worldwide as hidden hunger, is over two billion and also considered a public health problem. Therefore, this study aimed to evaluate biofortified cassava cultivars in relation to their physical-chemical composition, total carotenoids and to verify their sensory acceptance by schoolchildren. The study was conducted in Santa Maria, in the state of Rio Grande do Sul (RS), Brazil, with four cassava cultivars, two biofortified with yellow pulp (BRS 399 and BRS 396); two non-biofortified varieties: one with yellow pulp (“Gema de ovo”) and another with white pulp (“Vassourinha”). After cooking the roots, the nutritional composition (moisture, protein, lipid, ash, and total starch), the total carotenoid content, and the sensory acceptance of the roots by the children at school, in the countryside of Julio de Castilhos and Dilermando de Aguiar, was determined. The contents of moisture, protein, lipids, ash, and starch showed significant variations between the cultivars analyzed. The highest levels of total carotenoids were found in biofortified cultivars (12.85 µg g-1). In the non-biofortified cultivars, “Gema de ovo” and “Vassourinha”, the levels were 12.01 µg g-1 and 3.30 µg g-1, respectively. Sensory acceptance was 78.7%, demonstrating the potential for the insertion of biofortified roots in school meals.

Abstract This study evaluated the effects of the addition of encapsulated Lactobacillus acidophilus and green banana biomass on the characteristics of requeijão cremoso processed cheese during the storage period of 45 days under refrigeration. The elaborated requeijões cremosos processed cheeses included addition of encapsulated Lactobacillus acidophilus as probiotic source, and green banana biomass as partial fat substitute, in a total of five treatments. Analyses were performed to evaluate probiotic viability, microbiological conditions, centesimal composition (moisture, fat, fat in dry extract, protein, ashes, carbohydrates), water activity, resistant starch, colour, texture, lipid profile and sensory acceptability. The addition of green banana biomass and fat reduction resulted in changes in the centesimal composition of the cheeses, with their moisture indexes increased and protein decreased, the F1 formulation was the only one that can be considered as low fat content and was also the one that presented higher moisture. The yellowish white coloration of the formulations was altered as a function of the composition as well as the texture parameters, F1 and F3 treatments presented the greatest changes. Probiotic viability did not differ between formulations, it only changed over time.

RESUMO Objetivou-se avaliar o efeito dos níveis de caroço de algodão (0; 100; 200; 300 e 400 g Kg-1) na terminação de cordeiros sobre os parâmetros físicos e químicos da carne. Utilizou-se 45 cordeiros, machos, não castrados, distribuídos ao acaso em delineamento inteiramente casualizado com cinco tratamentos e nove repetições cada. Os valores de pH, não foram influenciados pelos níveis de caroço de algodão. Os teores de lipídios e cinzas apresentaram diferença significativa, mas não foram ajustados pelas equações lineares ou quadráticas. As variáveis perdas por descongelamento e coesividade aumentaram, e a atividade de água e capacidade de retenção de água diminuíram com o incremento de caroço de algodão na dieta. Já os tocoferóis, perdas por cocção, dureza e mastigabilidade foram ajustados através de equações quadráticas. Na avaliação instrumental de cor o L*, b* e h° demonstraram comportamento linear crescente. Logo a inclusão de até 400 g de caroço de algodão na dieta influencia a composição físico-química e na cor da carne produzida.

ABSTRACT The aim of this study was to evaluate the effect of different levels of cottonseed (0, 100, 200, 300 and 400 g Kg-1) on the physical and chemical parameters of the meat of finishing lambs. Forty-five uncastrated male lambs were distributed in a completely randomised design with five treatments of nine replications. The pH values were not influenced by the levels of cottonseed. The lipid and ash content showed significant differences, but were not adjusted by the linear or quadratic equations. Losses during thawing increased, as did cohesiveness, and water activity and water retention capacity decreased as the levels of cottonseed in the diet rose. Tocopherols, cooking loss, toughness and chewiness were adjusted using quadratic equations. In the instrumental colour evaluation, L*, b* and h° showed increasing linear behaviour. The inclusion of up to 400 g of cottonseed in the diet affects the physical-chemical composition and the colour of the produced meat.

Abstract Functional foods are those that bring benefits to human health beyond nutrition such as the prevention of cardiovascular diseases, allergies and intestinal problems. Among the functional foods highly consumed worldwide, yogurt is highlighted, especially in the probiotic and symbiotic versions. The aim of this study was to use the Jerusalem artichoke tuber flour ( Helianthus tuberosus L.) as a new prebiotic source for the development of functional yogurt. The identification and quantification of the fructans of the Jerusalem artichoke flour was performed and later yoghurt formulations were elaborated. These yogurts were submitted to analyses of centesimal composition, shelf life and sensorial evaluation. For the biological evaluation, a symbiotic yogurt formulation was chosen (the best accepted in sensorial evaluation) and included in the feed of New Zealand white rabbits, in the growth and final phase. The in vivo experiment had three distinct groups (control group, probiotic group and symbiotic group). At the end of 50 days of experiment, the animals were euthanized and submitted to the serological and cecal microbiota evaluation. Through this study, the prebiotic/bifidogenic capacity of Jerusalem artichoke flour was evidenced.

RESUMO: Estima-se que em 2050 a população mundial contará com cerca de 9 bilhões de pessoas, e, portanto, a necessidade de fontes alternativas de proteína é inevitável, uma vez que as fontes convencionais, como carne de gado, suínos e aves, não serão suficientes para suprir a demanda do crescimento populacional. A alimentação que inclui fontes alternativas de proteína, como os insetos, é uma realidade em países da América Latina, Ásia, Austrália, Europa e África. Esta pesquisa apresenta os resultados de um estudo exploratório que analisou o perfil alimentar de 1.619 consumidores das cinco regiões brasileiras (Norte, Nordeste, Centro-Oeste, Sudeste e Sul) quanto sua percepção, motivação e forma preferencial aos insetos comestíveis. Os dados foram analisados através de frequências cruzadas e expressos em porcentagem. Nossos resultados mostram que mulheres apresentam maior aversão ao consumo de insetos do que homens. Em geral, há preferência em consumir os insetos na forma de farinha, e inteiro para aqueles com mais familiaridade ao consumo de insetos. Majoritariamente, os consumidores brasileiros não tem opinião sobre a segurança em consumir insetos, contudo, em maiores níveis de escolaridade e a familiaridade os consumidores posicionam-se quanto à sua segurança.

ABSTRACT: It is estimated that by 2050 the world population will be 9 billion people; and therefore, the need for alternative sources of protein is inevitable, since conventional sources, such as beef, pork and poultry, will not be sufficient to meet the demand of population growth. Food that includes alternative sources of protein, such as insects, is a reality in countries of Latin America, Asia, Australia, Europe and Africa. This research presents the results of an exploratory study that analysed the food profile of 1,619 consumers in the five Brazilian regions (North, Northeast, Midwest, Southeast and South) as well as their perception, motivation and preferred form of edible insects. The data were analysed by cross-tabulation and expressed as frequencies. Our results show that women are more reluctant than men to consume insects. In general, there is a preference for consumption of insects in the form of flour. However, those with more familiarity with this type of consumption prefer the whole insect. Most Brazilian consumers have no opinion about the safety of consuming insects; however, consumers with higher levels of education and familiarity consider it safe.

Abstract Kefir is a product elaborated from the symbiotic fermentation of different microorganisms. The Kluyveromyces and Saccharomyces genera are the major representatives of the yeasts found in kefir microbiota. The only pobiotic yeast commercialized as an oral medication, is the Saccharomyces boulardii. The present work involved the microbiological quality examination of six kefir samples in the city of Santa Maria/RS, the yeasts isolation present in the samples and the identification of them by PCR (Polymerase chain reaction). Then, their probiotic potential was evaluated by in vitro technique. After that, microbiological analysis confirmed that kefir samples were suitable for consumption once the microbiological quality was established. Nineteen yeast strains were isolated from six different kefir samples; it was identified, by PCR analysis, but only three species were identified from these microorganisms in the present article: Saccharomyces cerevisiae, Hanseniospora uvarum and Kazachstania unispora. Nevertheless, by simulating the passage of isolated strains through the gastrointestinal environment, it was observed that they could not be considered probiotics. The results indicate that, in an isolated way, the yeast presents in kefir samples, in the city of Santa Maria, RS, can´t be considered probiotics according to the tests performed.

ABSTRACT The aim of this study was to modify the fatty acid profile of yogurt from sheep milk by the inclusion of different concentrations of palm oil into their diet. Thus, thirty-six sheep during lactation were separated in four groups with nine animals each, as described below: the group T0 (0%); the group T2 (inclusion of 2% of palm oil); the group T4 (inclusion of 4% of palm oil) and the group T6 (inclusion of 6% of palm oil). After 60 days of the supplementation, milk samples were collected and yogurt was produced, which was evaluated regarding the concentration of saturated fatty acids (SFA), monounsaturated fatty acids (MFA), and polyunsaturated fatty acids (PFA). A significant reduction (p<0.05) in most SFA and a significant increase (p<0.05) on MFA and PFA was observed in the yogurt of sheep supplemented with 4 and 6% of palm oil. Consequently, it is possible to conclude that palm oil supplementation exerts positive effects on yogurt, since it led to the reduction of undesirable fatty acids and increased fatty acids beneficial to human health.

No início dos anos 90, foi introduzido na cultura Brasileira o plantio da batata yacon, uma raiz tuberosa, que vem despertando grande interesse por parte dos pesquisadores, devido as suas propriedades nutricionais e tecnológicas. Essa raiz caracteriza-se principalmente por ser rica em frutanos e ácidos fenólicos, apresentando efeito prebiótico e antioxidante, respectivamente. Muitos estudos in vivo e in vitro já foram realizados, utilizando este tubérculo e seus derivados, comprovando suas atividades benéficas à saúde, além deter grande potencial na tecnologia do desenvolvimento de novos produtos alimentícios. A presente revisão objetivou agrupar alguns desses estudos, tanto na área da saúde como na da tecnologia de alimentos. O potencial prebiótico, redução do índice glicêmico e a melhora na saúde óssea são os efeitos que se destacam nas pesquisas in vivo. Percebe-se também uma tendência na elaboração de produtos de panificação utilizando-se a farinha de yacon, e os resultados apresentam-se satisfatórios, tanto nas propriedades tecnológicas quanto nas avaliações sensoriais

In the early nineties was introduced in Brazil the yacon potato planting. This tuberous roots have been attracting interest from researchers due to their nutritional and technological properties.Yacon is its mainly characterized by being rich in fructans and phenolic acids, with prebiotic and antioxidant effects, respectively. Many studies in vivo and in vitro were carried out with yacon and products produced from it, showing its health benefits, besides having great technological potential for developing new food products .This review aimed to outline some of these studies, in both health and food technology. The prebiotic potential, glycemic index reduction and improvement of bone health are the effects that stand out in the in vivo researches. It can be noted a trend for bakery products formulations using yacon flour, and the outcomes were positive for both technological properties as well as for sensory evaluations

Na produção de pães integrais de trigo no Brasil, são utilizadas mesclas de farinha integral (FI) com farinha refinada (FR), as quais diferem em sua composição. Assim, o objetivo deste estudo foi investigar as propriedades de pasta de mesclas de FI com FR. Foram analisadas amostras com 100% de FI, 100% de FR (controles) e mesclas de FI com FR nas proporções de 20, 40, 60 e 80%, em equipamento RVA (Rapid Visco Analyzer). A temperatura de pasta das amostras foi semelhante. A viscosidade máxima diminuiu proporcionalmente com o aumento de FI na mescla. Maiores valores de quebra e de viscosidade mínima à temperatura constante foram encontrados em farinhas com pico de viscosidade elevado. Os valores de viscosidade final e de tendência à retrogradação diminuíram à medida que aumentou o teor de FI na mescla. As propriedades de pasta das mesclas de FI com FR devem ser vistas de forma conjunta, pois afetam a produção de pão e o armazenamento. Os resultados mostraram também que o RVA pode ser usado para ajudar a formular mesclas de FI/FR com certas propriedades de pasta.

In whole wheat bread production in Brazil blends of whole flour (WF) with refined flour (RF) are used, which differ in their composition. Thus, the objective of this study was to investigate the pasting properties of WF with RF blends. Samples with 100% of WF, 100% of RF (controls) and blends of WF with RF in the proportions of 20, 40, 60 and 80% were analyzed, in a RVA (Rapid Visco Analyzer) equipment. The samples pasting temperatures were similar. The peak viscosity decreased proportionally with the increase of WF in blends. Higher values of breakdown and holding strength were found in flour with high viscosity peak. Final viscosity and setback values decreased as the content of WF in blends increased. The pasting properties of WF with RF blends must be seen in a combined way, because they affect bread production and storage. The results also showed that RVA can be used to help in formulating WF/RF blends with certain pasting properties.

Esta pesquisa foi conduzida com o objetivo de avaliar o efeito in vitro e in vivo do óleo de eucalipto (Corymbia citriodora) sobre o carrapato bovino (Rhipicephalus (Boophilus) microplus). Na experimentação in vitro, foi utilizado o grupo controle negativo e oito concentrações de óleo de eucalipto (0,5; 1; 2; 5; 10; 20; 50; 100%), em fêmeas ingurgitadas de carrapato. A eficácia de controle foi de 0; 30,5; 75,5; 91; 100; 100; 100; 100 e 100%, respectivamente. Para a experimentação in vivo, foram constituídos três grupos (controle negativo; óleo de eucalipto a 3,5% - nível estimado mediante análise de regressão, correspondendo a 95% de eficácia de controle do carrapato da pesquisa in vitro e amitraz a 0,025%), com dezoito vacas da raça Holandesa. Antes (média dos dias -3, -2,-1) e após a aplicação do produto (1, 2, 3, 5, 7, 10, 14, 21dias), foram contadas fêmeas ingurgitadas de carrapato. A eficácia de controle foi de 0; 96,4 e 69%, respectivamente, 21 dias após o tratamento. Na 1ª e na 2ª ordenha após a aplicação dos tratamentos, foram avaliadas variáveis fisiológicas e coletadas amostras de leite para avaliar as propriedades organolépticas no leite e no iogurte (controle negativo x tratamento fitoterápico). O teste de aceitação sensorial do leite e das variáveis fisiológicas avaliadas foram similares entre os tratamentos.

This research was aimed at evaluating in vitro and in vivo effects of eucalyptus (Corymbia citriodora) oil on cattle ticks (Rhipicephalus (Boophilus) microplus). Negative control group and eight concentrations of eucalyptus oil (0.5; 1; 2; 5; 10; 20; 50; 100%), were used on in vitro trials with engorged female ticks. The efficacy of control ticks was 0; 30.5; 75.5; 91; 100; 100; 100; 100 and 100%, respectively. At the in vivo trial eighteen Holstein cows were allocated to three groups (negative control, eucalyptus oil at 3.5% - level estimated by regression analysis, accounting for 95% efficacy of control ticks on in vitro trial and amitraz at 0.025%). Engorged female ticks were counted before (mean of days -3, -2, -1) and after treatment (1, 2, 3, 5, 7, 10, 14, 21 days). The efficacy control tick was 0; 96.4 and 69%, respectively, at 21 days after treatment. On the 1st and 2nd milked after treatments, physiologic variables were evaluate and milk samples were collected to evaluate organoleptic properties in milk and yoghurt (negative control x phytotherapic treatment). Similar results were found with sensorial acceptance test of milk and physiologic variables.